In this tutorial, we describe a method for visualizing and quantitating neurite outgrowth in co culture with astrocytes, utilizing a novel fluorescent label for neuronal processes and the IncuCyte ZOOM live cell imaging system. The fluorescent reagent, NeuroLight Red, specifically labels both primary neurons and human iPSC derived neurons when grown in co culture with astrocytes, allowing for quantitation using the IncuCyte NeuroTrack software. This kinetic, 96 well assay method provides dynamic information on neurite outgrowth that is not available from monoculture or end point experiments and enables pharmacological studies of neuroactive agents..
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